SI.LINE_FORMAT_VERSION = 1 SI.TIFF_FORMAT_VERSION = 4 SI.VERSION_COMMIT = '6e521b3bedc8d8d87d761189b679ea68aeefd0ff' SI.VERSION_MAJOR = '2019b' SI.VERSION_MINOR = '1' SI.acqState = 'grab' SI.acqsPerLoop = 1 SI.extTrigEnable = false SI.hBeams.beamCalibratedStatus = false SI.hBeams.directMode = false SI.hBeams.enablePowerBox = false SI.hBeams.extPowerScaleFnc = [] SI.hBeams.flybackBlanking = true SI.hBeams.interlaceDecimation = 1 SI.hBeams.interlaceOffset = 0 SI.hBeams.lengthConstants = Inf SI.hBeams.powerBoxEndFrame = Inf SI.hBeams.powerBoxStartFrame = 1 SI.hBeams.powerBoxes.rect = [0.25 0.25 0.5 0.5] SI.hBeams.powerBoxes.powers = NaN SI.hBeams.powerBoxes.name = '' SI.hBeams.powerBoxes.oddLines = true SI.hBeams.powerBoxes.evenLines = true SI.hBeams.powerLimits = 100 SI.hBeams.powers = 25 SI.hBeams.pzAdjust = false SI.hBeams.pzCustom = {[]} SI.hBeams.tfExtForceStreaming = 0 SI.hChannels.channelAdcResolution = {14 14} SI.hChannels.channelDisplay = [1;2] SI.hChannels.channelInputRange = {[-1 1] [-1 1]} SI.hChannels.channelLUT = {[8083 8191] [-95 355]} SI.hChannels.channelMergeColor = {'green';'red'} SI.hChannels.channelName = {'Channel 1' 'Channel 2'} SI.hChannels.channelOffset = [-4009 41] SI.hChannels.channelSave = [1;2] SI.hChannels.channelSubtractOffset = [true true] SI.hChannels.channelType = {'stripe' 'stripe'} SI.hChannels.channelsActive = [1;2] SI.hChannels.channelsAvailable = 2 SI.hChannels.loggingEnable = 1 SI.hConfigurationSaver.cfgFilename = 'C:\Users\Laser\Documents\MATLAB\Didrik.cfg' SI.hConfigurationSaver.usrFilename = 'C:\Users\Laser\Documents\MATLAB\Didrik.usr' SI.hCycleManager.cycleIterIdxTotal = 0 SI.hCycleManager.cyclesCompleted = 0 SI.hCycleManager.enabled = false SI.hCycleManager.itersCompleted = 0 SI.hCycleManager.totalCycles = 1 SI.hDisplay.autoScaleSaturationFraction = [0.1 0.01] SI.hDisplay.channelsMergeEnable = 1 SI.hDisplay.channelsMergeFocusOnly = false SI.hDisplay.displayRollingAverageFactor = 1 SI.hDisplay.displayRollingAverageFactorLock = false SI.hDisplay.enableScanfieldDisplays = false SI.hDisplay.lineScanHistoryLength = 1000 SI.hDisplay.renderer = 'auto' SI.hDisplay.scanfieldDisplayColumns = 5 SI.hDisplay.scanfieldDisplayRows = 5 SI.hDisplay.scanfieldDisplayTilingMode = 'Auto' SI.hDisplay.scanfieldDisplays.enable = false SI.hDisplay.scanfieldDisplays.name = 'Display 1' SI.hDisplay.scanfieldDisplays.channel = 1 SI.hDisplay.scanfieldDisplays.roi = 1 SI.hDisplay.scanfieldDisplays.z = 0 SI.hDisplay.selectedZs = [] SI.hDisplay.showScanfieldDisplayNames = true SI.hDisplay.volumeDisplayStyle = '3D' SI.hFastZ.actuatorLag = 0 SI.hFastZ.discardFlybackFrames = false SI.hFastZ.enable = false SI.hFastZ.enableFieldCurveCorr = false SI.hFastZ.flybackTime = 0 SI.hFastZ.hasFastZ = false SI.hFastZ.nonblockingMoveInProgress = false SI.hFastZ.numDiscardFlybackFrames = 0 SI.hFastZ.positionAbsoluteRaw = NaN SI.hFastZ.positionTarget = 0 SI.hFastZ.positionTargetRaw = 0 SI.hFastZ.volumePeriodAdjustment = -0.0006 SI.hFastZ.waveformType = 'sawtooth' SI.hFastZ.zAlignment = [] SI.hIntegrationRoiManager.enable = false SI.hIntegrationRoiManager.enableDisplay = true SI.hIntegrationRoiManager.integrationHistoryLength = 1000 SI.hIntegrationRoiManager.postProcessFcn = @scanimage.components.integrationRois.integrationPostProcessingFcn SI.hMotionManager.correctionBoundsXY = [-5 5] SI.hMotionManager.correctionBoundsZ = [-50 50] SI.hMotionManager.correctionDeviceXY = 'galvos' SI.hMotionManager.correctionDeviceZ = 'fastz' SI.hMotionManager.correctionEnableXY = false SI.hMotionManager.correctionEnableZ = false SI.hMotionManager.correctorClassName = 'scanimage.components.motionCorrectors.SimpleMotionCorrector' SI.hMotionManager.enable = false SI.hMotionManager.estimatorClassName = 'scanimage.components.motionEstimators.SimpleMotionEstimator' SI.hMotionManager.motionHistoryLength = 100 SI.hMotionManager.motionMarkersXY = zeros(0,2) SI.hMotionManager.resetCorrectionAfterAcq = true SI.hMotionManager.zStackAlignmentFcn = @scanimage.components.motionEstimators.util.alignZRoiData SI.hMotors.axesPosition = [44674.1 26421.9 18414.7] SI.hMotors.azimuth = 0 SI.hMotors.backlashCompensation = [0 0 0] SI.hMotors.elevation = 0 SI.hMotors.errorMsg = {''} SI.hMotors.errorTf = false SI.hMotors.isAligned = false SI.hMotors.isRelativeZeroSet = true SI.hMotors.minPositionQueryInterval_s = 0.001 SI.hMotors.motorPosition = [662.94 -422.91 -867.727] SI.hMotors.moveInProgress = false SI.hMotors.moveTimeout_s = 10 SI.hMotors.samplePosition = [-662.94 422.91 867.727] SI.hMotors.simulatedAxes = [false false false] SI.hMotors.userDefinedPositions = [] SI.hPhotostim.allowMultipleOutputs = false SI.hPhotostim.autoTriggerPeriod = 0 SI.hPhotostim.compensateMotionEnabled = true SI.hPhotostim.completedSequences = 0 SI.hPhotostim.laserActiveSignalAdvance = 0.001 SI.hPhotostim.lastMotion = [0 0] SI.hPhotostim.logging = false SI.hPhotostim.monitoring = false SI.hPhotostim.monitoringSampleRate = 9000 SI.hPhotostim.nextStimulus = 1 SI.hPhotostim.numOutputs = 0 SI.hPhotostim.numSequences = Inf SI.hPhotostim.sequencePosition = 1 SI.hPhotostim.sequenceSelectedStimuli = [] SI.hPhotostim.status = 'Offline' SI.hPhotostim.stimImmediately = false SI.hPhotostim.stimSelectionAssignment = [] SI.hPhotostim.stimSelectionDevice = '' SI.hPhotostim.stimSelectionTerms = [] SI.hPhotostim.stimSelectionTriggerTerm = [] SI.hPhotostim.stimTriggerTerm = 1 SI.hPhotostim.stimulusMode = 'onDemand' SI.hPhotostim.syncTriggerTerm = [] SI.hPhotostim.zMode = '2D' SI.hPmts.autoPower = [] SI.hPmts.bandwidths = [] SI.hPmts.gains = [] SI.hPmts.names = [] SI.hPmts.offsets = [] SI.hPmts.powersOn = [] SI.hPmts.tripped = [] SI.hRoiManager.forceSquarePixelation = true SI.hRoiManager.forceSquarePixels = true SI.hRoiManager.imagingFovDeg = [0.156942 -0.402028;-0.127467 -0.402028;-0.127467 0.44879;0.156942 0.44879] SI.hRoiManager.imagingFovUm = [2.35414 -6.03042;-1.91201 -6.03042;-1.91201 6.73184;2.35414 6.73184] SI.hRoiManager.linePeriod = 0.0040008 SI.hRoiManager.linesPerFrame = 512 SI.hRoiManager.mroiEnable = false SI.hRoiManager.pixelsPerLine = 512 SI.hRoiManager.scanAngleMultiplierFast = 1 SI.hRoiManager.scanAngleMultiplierSlow = 1 SI.hRoiManager.scanAngleShiftFast = 0 SI.hRoiManager.scanAngleShiftSlow = 0 SI.hRoiManager.scanFramePeriod = 0.0040008 SI.hRoiManager.scanFrameRate = 249.95 SI.hRoiManager.scanRotation = 0 SI.hRoiManager.scanType = 'line' SI.hRoiManager.scanVolumeRate = 249.95 SI.hRoiManager.scanZoomFactor = 13 SI.hScan2D.beamClockDelay = 1.5e-06 SI.hScan2D.beamClockExtend = 0 SI.hScan2D.bidirectional = true SI.hScan2D.channelOffsets = [-4009 41] SI.hScan2D.channels = {} SI.hScan2D.channelsAdcResolution = 14 SI.hScan2D.channelsAutoReadOffsets = true SI.hScan2D.channelsAvailable = 2 SI.hScan2D.channelsDataType = 'int16' SI.hScan2D.channelsFilter = 'None' SI.hScan2D.channelsInputRanges = {[-1 1] [-1 1]} SI.hScan2D.channelsSubtractOffsets = [true true] SI.hScan2D.fillFractionSpatial = 0.9 SI.hScan2D.fillFractionTemporal = 0.9 SI.hScan2D.flybackTimePerFrame = 0.001 SI.hScan2D.flytoTimePerScanfield = 0.001 SI.hScan2D.fovCornerPoints = [-7.5 -7.5;7.5 -7.5;7.5 7.5;-7.5 7.5] SI.hScan2D.hasResonantMirror = false SI.hScan2D.hasXGalvo = true SI.hScan2D.laserTriggerDebounceTicks = 0 SI.hScan2D.laserTriggerSampleMaskEnable = false SI.hScan2D.laserTriggerSampleWindow = [0 1] SI.hScan2D.linePhase = 0.0001248 SI.hScan2D.lineScanFdbkSamplesPerFrame = [] SI.hScan2D.lineScanNumFdbkChannels = 2 SI.hScan2D.lineScanSamplesPerFrame = 5001 SI.hScan2D.logAverageFactor = 1 SI.hScan2D.logFileCounter = 12 SI.hScan2D.logFilePath = 'D:\Kristin\12112020\AQP4KO_4' SI.hScan2D.logFileStem = 'file' SI.hScan2D.logFramesPerFile = Inf SI.hScan2D.logFramesPerFileLock = false SI.hScan2D.logOverwriteWarn = false SI.hScan2D.maskDisableAveraging = false SI.hScan2D.maxSampleRate = 2.5e+06 SI.hScan2D.name = 'LinScanner' SI.hScan2D.nominalFovCornerPoints = [-7.5 -7.5;7.5 -7.5;7.5 7.5;-7.5 7.5] SI.hScan2D.pixelBinFactor = 4 SI.hScan2D.recordScannerFeedback = false SI.hScan2D.sampleRate = 1.25e+06 SI.hScan2D.sampleRateCtl = 416667 SI.hScan2D.sampleRateFdbk = 125000 SI.hScan2D.scanMode = 'linear' SI.hScan2D.scanPixelTimeMaxMinRatio = 1 SI.hScan2D.scanPixelTimeMean = 3.2e-06 SI.hScan2D.scannerFrequency = NaN SI.hScan2D.scannerToRefTransform = [1 0 0;0 1 0;0 0 1] SI.hScan2D.scannerType = 'GG' SI.hScan2D.settleTimeFraction = 0 SI.hScan2D.simulated = false SI.hScan2D.stripingEnable = true SI.hScan2D.trigAcqEdge = 'rising' SI.hScan2D.trigAcqInTerm = '' SI.hScan2D.trigNextEdge = 'rising' SI.hScan2D.trigNextInTerm = '' SI.hScan2D.trigNextStopEnable = false SI.hScan2D.trigStopEdge = 'rising' SI.hScan2D.trigStopInTerm = '' SI.hStackManager.actualNumSlices = 1 SI.hStackManager.actualNumVolumes = 1 SI.hStackManager.actualStackZStepSize = [] SI.hStackManager.arbitraryZs = [0 1] SI.hStackManager.centeredStack = false SI.hStackManager.closeShutterBetweenSlices = false SI.hStackManager.enable = false SI.hStackManager.framesPerSlice = 310000 SI.hStackManager.numFramesPerVolume = 1 SI.hStackManager.numFramesPerVolumeWithFlyback = 1 SI.hStackManager.numSlices = 1 SI.hStackManager.numVolumes = 1 SI.hStackManager.stackActuator = 'motor' SI.hStackManager.stackDefinition = 'uniform' SI.hStackManager.stackEndPower = [] SI.hStackManager.stackFastWaveformType = 'sawtooth' SI.hStackManager.stackMode = 'slow' SI.hStackManager.stackReturnHome = true SI.hStackManager.stackStartPower = [] SI.hStackManager.stackZEndPos = [] SI.hStackManager.stackZStartPos = [] SI.hStackManager.stackZStepSize = 1 SI.hStackManager.useStartEndPowers = true SI.hStackManager.zPowerReference = 795.337 SI.hStackManager.zs = 795.337 SI.hStackManager.zsRelative = 0 SI.hUserFunctions.userFunctionsCfg = [] SI.hUserFunctions.userFunctionsUsr = [] SI.hWSConnector.communicationTimeout = 5 SI.hWSConnector.enable = false SI.hWaveformManager.optimizedScanners = {} SI.imagingSystem = 'LinScanner' SI.loopAcqInterval = 10 SI.objectiveResolution = 15 { "RoiGroups": { "imagingRoiGroup": { "ver": 1, "classname": "scanimage.mroi.RoiGroup", "name": "Line Scanning ROI Group", "UserData": null, "roiUuid": "9F3F014EDA603F51", "roiUuiduint64": 1.147489181e+19, "rois": { "ver": 1, "classname": "scanimage.mroi.Roi", "name": "", "UserData": null, "roiUuid": "1E3F6C12D6FCC3CE", "roiUuiduint64": 2.179579573e+18, "zs": 795.337, "scanfields": { "ver": 1, "classname": "scanimage.mroi.scanfield.fields.StimulusField", "name": "", "UserData": null, "roiUuid": "496D8350FDBEDF99", "roiUuiduint64": 5.291029521e+18, "centerXY": [0.01473766859,0.02338067099], "sizeXY": [-0.2844093863,0.8508179098], "rotationDegrees": 0, "enable": 1, "stimulusFunction": "scanimage.mroi.stimulusfunctions.line", "stimparams": [ "revolutions", 5 ], "duration": 0.004, "repetitions": 1, "powers": null, "zSpan": 0, "beamsFunction": "scanimage.mroi.stimulusbeamfunctions.beamPowers", "slmPattern": null }, "discretePlaneMode": 0, "powers": null, "pzAdjust": null, "Lzs": null, "interlaceDecimation": null, "interlaceOffset": null, "enable": 1 } }, "photostimRoiGroups": null, "integrationRoiGroup": { "ver": 1, "classname": "scanimage.mroi.RoiGroup", "name": "", "UserData": null, "roiUuid": "E4123C4DB2F6B12D", "roiUuiduint64": 1.643426429e+19, "rois": { "_ArrayType_": "double", "_ArraySize_": [1,0], "_ArrayData_": null } } } }