Folder name: Plate name _running repetition (a,b,c)_ variant of seed (manual or nucleus) Folder name: Plate name_ running repetition (a,b,c)_ variant of seed for automatic segmentation with CellSegm (manual or nucleus) File name: siRNA target gene plus its sequence number or transfected vector_ coordinates on 24 well plate_ txt. File contents: channel classification in correct order, date of execution of experiment, transfection target (donor, acceptor, both or double both) if manual, a folder contains the manual marks for nuclei in red and for background in blue manual_nucleus/ WGA-AF488_DiD_transmission 2012_03_06, Double both